Apparently irreversible GTP hydrolysis attends tubulin self-assembly
نویسندگان
چکیده
منابع مشابه
Incorporation of tubulin subunits into dimers requires GTP hydrolysis.
A toroid multisubunit complex of 800-900 kDa has been implicated in assisting protein folding of at least two cytoplasmic proteins, actin and tubulin. This process is dependent on the presence of magnesium ions and ATP hydrolysis. In vitro translation of cDNAs encoding different alpha- and beta-tubulin isotypes also gives rise to the formation of complexes of about 300 kDa. These complexes have...
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We study the stochastic dynamics of growth and shrinkage of single actin filaments or microtubules taking into account insertion, removal, and ATP/GTP hydrolysis of subunits. The resulting phase diagram contains three different phases: two phases of unbounded growth: a rapidly growing phase and an intermediate phase, and one bounded growth phase. We analyze all these phases, with an emphasis on...
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GTP hydrolysis accompanies addition of tubulin to microtubules. I find that hydrolysis is a requirement for the opposite-end assembly/disassembly of microtubules and consequent subunit treadmilling from one end to the other of the polymer. Neither GDP nor guanosine 5'-[beta, gamma-imido]triphosphate allows or participates in the treadmilling reaction. Therefore, there is a requirement for hydro...
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FtsZ forms a cytokinetic ring, designated the Z ring, that directs cytokinesis in prokaryotes. It has limited sequence similarity to eukaryotic tubulins and, like tubulin, it has GTPase activity and the ability to assemble into various structures including protofilaments, bundles and minirings. By using both electron microscopy and sedimentation, we demonstrate that FtsZ from Escherichia coli u...
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A method is given for preparing tubulin with 1 mol of exchangeably bound [gamma-32P]GTP/mol of 6 S dimer. Bovine tubulin is shown to hydrolyze 1 mol of GTP/mol of 6 S dimer added to assembling microtubules at 37 degrees. Hydrolysis and assembly occur at the same rate and to the same extent. When microtubule-associated proteins (MAPs) are removed, both hydrolysis and assembly fail to occur. Read...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1990
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1990.tb19150.x